A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii | Communications Biology - Nature.com

A high-throughput workflow involving a streamlined genome-wide screen improves protein production in the non-conventional yeast, Komagataella phaffii, following adaptive laboratory evolution.

Strains and media condition The wild-type K. phaffii strain CBS7435 (NRRL-Y11430) was used in the REMI screen. The K. phaffii strain dnl4his433 was used for accurate gene disruptions in the effectiv… [+20443 chars]

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