Mechanism of replication origin melting nucleated by CMG helicase assembly - Nature.com

Cryo-electron microscopy structures of the sequential assembly of the CMG replicative helicase on a chromatinized origin of replication provide insights into the mechanism through which DNA melting is initiated by ATP binding.

Cloning, expression and purification ORC, Cdc6, Mcm27Cdt1, DDK, CDK, Sld2, Sld3Sld7, Cdc45, Dpb11, Pol , Pol exo-, Pol , TopoI, Mcm10 and yeast histone octamer were purified on the basis of previous… [+23767 chars]

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