Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells | Communications Biology - Nature.com

A small-size engineered enzyme, ultraID, is presented for proximity-dependent biotinylation, that shows efficient labeling in mammalian cell culture, E. coli and S. cerevisiae.

microID, a small size biotin ligase derived from BioID2 We previously developed a split-BioID assay in which BioID is split into two inactive fragments that reassemble an active enzyme when fused to… [+31924 chars]

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