In vivo dissection of a clustered-CTCF domain boundary reveals developmental principles of regulatory insulation - Nature.com

Genetically dissecting the Epha4–Pax3 topological boundary in mice shows that divergent CTCF binding sites (CBSs) are not essential for insulation and that chromatin loops in nonconvergently oriented CBSs can be driven by a loop interference mechanism.

A genetic setup to investigate boundary function in vivo We previously demonstrated that a 150-kilobase (kb) region, the EP boundary, is sufficient to segregate the regulatory activities of the Epha… [+33714 chars]

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