Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs - Nature.com

Genome-wide CRISPR knockout and activation screens in Calu-3 lung cells and Caco-2 colorectal cells identify host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs.

Plasmids and constructs The pLX_311-Cas9 (Addgene, 96924), pRDA_174 (Addgene, 136476), pXPR_BRD109 (lenti dCAS-VP64_Blast49, Addgene, 61425), which express Cas9, Cas12a and dCas9-VP64, respectively,… [+23591 chars]

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