Architecture and self-assembly of the jumbo bacteriophage nuclear shell - Nature.com

The nucleus-like compartment formed in bacteria during infection by jumbo phage 201phi2-1 is composed of the bacteriophage protein chimallin, which can self-assemble into closed compartments in vitro.

Bacterial and phage growth conditions P. chlororaphis 200-B21 was grown on hard agar (HA) medium (10 g l1 Bacto-Tryptone, 5 g l1 NaCl and 10 g l1 agar in distilled water) at 30°C. 201phi2-1 lysates … [+38101 chars]

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